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Автор: sveta on . Posted in 2019_02(30)

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UDC 663.938.4:658.62.018   DOI: https://doi.org/10.31617/tr.knute.2019(30)03
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ORCID: 0000-0002-9443-2941
  Doctor of Sciences (Technical), Associate Professor at the Department
of Technology and Organization of Restaurant Business,
Kyiv National University of Trade and Economics
19, Kyoto str., Kyiv, 02156, Ukraine
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ORCID: 0000-0003-3090-9250
  PhD in Technical Sciences, Associate Professor,
Associate Professor at the Department of Engineeringand Technical Disciplines, Kyiv National University of Trade and Economics
19, Kyoto str., Kyiv, 02156, Ukraine
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ORCID: 0000-0003-4140-3386
  PhD in Technical Sciences, Associate Professor,
Head of the Department of Engineering and Technical Disciplines,
Kyiv National University of Trade and Economics
19, Kyoto str., Kyiv, 02156, Ukraine


Background. Determination of the sizes and forms of particles – elements of the dispersed phase, has a priority, since these parameters determine many of the physical and technological properties of the bulk product. The most common method of dispersion analysis of the investigated system is to determine the size, number and shape of particles by ready-made photos of the microstructure.
The aim of this article is to test equipment and software for disperse analysis of bulk materials on the example of ground coffee using optical digital microscopes.
Materials and methods. The study used digital microscopes 500xSDM, CL PC camera 4.5, BW-400X, which are included in the price range 2500–5000 UAH. All devices are not equipped with an eyepiece or their own display, have LED direct illumination and resolution matrix from 3 to 8 Mp.
The TM Lavazza coffee was investigated, from which four different samples were selected from different parts of the consumer packaging. Five photos of the microstructure have been made. The dispersion analysis of the coffee samples was made using software provided by microscopic manufacturers. The sizes of the particles of the coffee ground by the photos of the microstructure were determined. The photos were made by the method of "dark field" and "light field".
Results. The matrixes used in the microscopy study let you to make photos with sizes from 640´ 480 to 3264´ 2448 pixels and a resolution of 0.3 to 8 megapixels. Since the resolution of the matrix in the combination with the multiplicity of the lens determines the degree of magnification of the digital microscope, small particles of ground coffee have been photographed at the highest resolution.
The research results had been exported to MS Excel by software to automate the calculation of the average linear particle size of the disperse system. The analysis results of each microstructure photo were stored in a separate file. When the scale of the analyzed photo was reset, a new calibration was performed or one of the saved templates was used.
Overall, the results of the study indicate that the distribution of ground coffee particles is reproduced with a deviation of 1.5 % for all investigated devices using standard software and is best described by a polynomial third-order equation.
Conclusion. It was determined that the disperse composition of the sample of ground coffee with a particle size of 20–250 μm, determined using 500xSDM, CL PC camera 4.5, BW-400X microscopes in the software environment Cooling Tech 4.5, Micro-Measure Tool, Image Tool differed within 1.5 %, indicating the reliability of the results.
An algorithm for processing the results of the dispersion analysis of bulk products has been developed, which makes it possible to obtain reliable results of the study.

Keywords: optical microscope, digital USB-microscope, automation of histological analysis, dispersion analysis, ground coffee.


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